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Diabetes Genome Anatomy Project

Joslin Diabetes Center Harvard Medical School Dana-Farber Cancer Institute Children's Hospital Boston Whitehead Institute UMASS Medical School

Resources > Protocols


Protocols

Project 1: Normal and Morbid Anatomy of Gene Expression in Mice

Project 2: Identifying the underlying alterations in gene expression which result in type 2 diabetes

Project 3: Anatomy of Gene Expression in Insulin Resistant States

Project 4: Information of levels, modification states and subcellular distribution of proteins in cells and tissues

Project 5B: Genetics of Adipose-Regulated Genes in Insulin-Resistance and Type 2 Diabetes

Project 7: Interfacing Small Molecules with the Diabetes Genome Anatomy Project

Project 8: Signal-dependent Interactions in Insulin Action

  • Project_8_Mann.doc contains:
    • Culture of brown adipocytes (BAT) in SILAC Medium and Insulin induction
    • Immunoprecipitation
    • In Gel digestion
    • Sample preparation after "in gel" digestion
    • HPLC system and mass spectrometer

Informatics Core

  • Relevance networks protocol - Introduction to Relevance Networks by Atul Butte, M.D., Ph.D.
  • Existing methods protocol - Introduction to existing methods in clustering microarray data by Atul Butte, M.D., Ph.D.
  • Amia Tutorial 2003 - Introduction to Bioinformatics tutorial slides presented at American - Medical Informatics Association 2003 meeting by Atul Butte, M.D., Ph.D.


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